Fibrosis: Methods and Protocols (Methods in Molecular Biology, Band 1627)Gebundene Ausgabe
Methods and Protocols Gebundenes Buch This volume describes state-of-the-art protocols that serve as "recipes" for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis in the lungs, skin, liver and heart, and generating transgenic mouse models. Part I I discusses cell culture systems, where the chapters explore cell types important for the development of fibrosis. Part I I I looks at the purification, quantification, and analysis of the E C M proteins, and Part I V describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy ( A F M). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Fibrosis: Methods and Protocols is a valuable resource aimed at outstanding quality and repeatability of research experiments in the fibrosis field.
|